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An anti-mouse macrophage monoclonal antibody reacting with T-derived leukaemic cells.
- Source :
- Immunology; Jul84, Vol. 52 Issue 3, p539-546, 8p
- Publication Year :
- 1984
-
Abstract
- Hybridomas secreting anti-mouse macrophage antibodies were obtained by fusing a routine plasmacytoma with lymphocytes of a rat immunized against mouse macrophages. An IgM, monoclonal antibody (3 A 35) reacted with mouse monocytes, macrophages and polymorphonuclear leucocytes. It did not bind appreciably to erythrocytes, platelets and unstimulated T or B lymphocytes. However, 3 A 35 bound to various murine T-derived leukaemic cells and to a small proportion of Con A-stimulated thymocytes. Cross absorption experiments confirmed the existence of a common antigenic determinant on macrophages and leukaemic cells. The possiblility that 3 A 35 identified a previously described antigen common to macrophages and normal or leukaemic T cells was investigated. The antibody was tested against thymocytes and macrophages of various mouse strains, some congenic for H-2 or Tla. The 3 A 35-detected antigen was found to be different from Ly5, Tla and Qa and did not represent a I-J encoded allotypic specificity. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOCLONAL antibodies
MACROPHAGES
LEUCOCYTES
ERYTHROCYTES
IMMUNOGLOBULINS
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 52
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13512775