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Expression of immunoglobulin-cross-reactive molecules by neoplastic human T cells I. SURFACE DETECTION AND ISOLATION OF MOLECULES REACTIVE WITH CHICKEN ANTI-F(ab′)2, ANTI-α AND ANTI-μ ANTIBODIES.
- Source :
- Immunology; Nov83, Vol. 50 Issue 3, p447-459, 13p
- Publication Year :
- 1983
-
Abstract
- There is evidence that the T-cell antigen receptor is immunoglobulin-related and that normal human T cells express surface determinants which cross-react with chicken anti-F(ab')<subscript>2</subscript>, anti-α and anti-μ antibodies. Surface marker study of six neoplastic human T cell lines suggested that F(ab')<subscript>2</subscript>- and μ-cross-reactive determinants may be expressed independently of <subscript>2</subscript>-cross-reactive determinants. F(ab')<subscript>2</subscript>-cross-reactive materials-F(ab')<subscript>2</subscript>-CRMs--were then isolated from several T-cell lines and analysed by reverse passive haemagglutination and in immunoprecipitation experiments by polyacrylamide disc gel electrophoresis in buffers containing sodium dodecyl sulphate (SDS-PAGE) under reducing conditions. It was shown that MOLT-4- and CCRF-CEM-derived F(ab')<subscript>2</subscript>-CRMs differ serologically and that α- are indeed expressed independently of F(ab')<subscript>2</subscript>- and μ-cross-reactive determinants. The F(ab')<subscript>2</subscript>-CRMs were shown to be T cell-derived, to express determinants similar to or identical with F(ab')<subscript>2</subscript>-cross-reactive determinants expressed in normal T-cell membranes and to correspond closely to T-cell antigen binding molecules. Evidence was also obtained that the two F(ab')<subscript>2</subscript>-CRMS differ from one another in molecular weight. SDS-PAGE under non-reducing conditions of MOLT-4- and CCRF-CEM-derived F(ab')<subscript>2</subscript>-CRMs identified major components of mol. wt. 135,000 and mol. wt. 330,000 respectively. Immunoprecipitation of <superscript>125</superscript>I-labelled F(ab')<subscript>2</subscript>-CRMs by chicken anti-F(ab')<subscript>2</subscript> followed by SDS-PAGE under reducing conditions identified major components of mol. wt. 80,000 and mol. wt. of 51,000 from CCRF-CEM-derived F(ab')<subscript>2</subscript>-CRMs and tentatively identified components of mol. wt. 52,000 and mol. wt. 23,000 from MOLT-4- derived F(ab')<subscript>2</subscript>-CRMs. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGLOBULINS
T cells
TUMORS
ANTIGENS
GEL electrophoresis
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 50
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13522378