Role of neutrophil extracellular traps in chronic kidney injury induced by bisphenol-A.

Bisphenol-A (BPA) is a common environmental pollutant, and expo sure to it is associated with proteinuria and may predict the progression of chronic kid ney disease; however, the mechanism is not clear. Neutrophil extracellular traps (NET s) are a DNA skeleton coated with various proteases, and it is associated with variou s types of autoimmune nephritis. In this study, we examine whether NETs is involved i n BPA-induced chronic kidney injury. In vivo, BPA exposure resulted in impaired renal function and altered renal morphology, including glomerular mesangial matrix expansion and increased renal interstitial fibroblast markers. Meanwhile, more dsDNA can be de tected in the serum, and the NETs-associated proteins, MPO and citH3 were deposited in the renal system. In vitro, BPA and NETs treatment caused podocyte injury, a loss of mark er proteins and disorder in the actin skeleton. After NETs inhibition via D Nase administration, BPA-induced injuries were significantly relieved. In conclusion, the increase of NETosis in circulation and the renal system during BPA exposure suggest s that NETs may be involved in BPA-induced chronic kidney injury. [ABSTRACT FROM AUTHOR]