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CD3+ T cells in severe combined immunodeficiency (scid) mice VI. RESCUE OF SCID-DERIVED, IGM-PRODUCING B CELLS BY TRANSFER OF CD4+ CD8– T CELLS FROM VARIOUS LYMPHOID ORGANS.
- Source :
- Immunology; Oct92, Vol. 77 Issue 2, p157-164, 8p
- Publication Year :
- 1992
-
Abstract
- Intravenous injection of purified CD4<superscript>+</superscript> CD8<superscript>-</superscript> T cells from thymus, spleen or lymph nodes of adult dm2 donor mice (H-2<superscript>d</superscript>, L<superscript>d-</superscript>, IgM<superscript>a</superscript>) into young C. B-17 scid/scid (scid) mice (H-2<superscript>d</superscript>, L<superscript>d+</superscript>, IgM<superscript>b</superscript>) partially and selectively reconstituted the splenic CD4<superscript>+</superscript> T-cell compartment of recipient scid mice. This was demonstrated by cytofluorographic analyses, histological examinations, growing donor-derived CD4<superscript>+</superscript> T-cell lines in vitro from spleens of transplanted scid mice, and serial passage of L<superscript>d-</superscript>, CD3<superscript>+</superscript> CD4<superscript>+</superscript> CD8<superscript>-</superscript> T-cell lines through young scid recipients for more than 1 year. Host-derived IgM<superscript>b</superscript> appeared in sera of all CD4<superscript>+</superscript> T-cell-transplanted young scid mice, while none of the sex- and age-matched, non-transplanted control scid mice was Ig<superscript>+</superscript>. B-cell leakiness was most efficiently induced by transfer of low numbers of adult CD4<superscript>+</superscript> CD8<superscript>-</superscript> thymocytes: transfer of 10³ purified CD4<superscript>+</superscript> CD8 thymocytes efficiently rescued scid-derived B cells, while transfer of 2 × 10<superscript>7</superscript> non-fractionated thymocytes from the same donor mouse was inefficient. Serum antibodies of scid mice with T-cell induced B-cell leakiness stained congenic 'double-positive' (CD4<superscript>+</superscript> CD8<superscript>+</superscript>) thymocytes and immunoprecipitated protein bands with an apparent MW of 14,000, 28,000, 43,000, 65,000 and 80,000 from <superscript>125</superscript>I-labelled thymocytes. These data indicate that repopulation of peripheral lymphoid organs with CD4<superscript>+</superscript> T cells is always accompanied by partial co-reconstitution of the B-cell system, and raises the question of the interdependence of these two lymphocyte subsets. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
THYMUS
CELL lines
IMMUNOGLOBULIN M
CD antigens
B cells
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 77
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13603693