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Subsets of null and γδ T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies.
- Source :
- Immunology; Oct92, Vol. 77 Issue 2, p219-227, 9p
- Publication Year :
- 1992
-
Abstract
- Rat monoclonal antibodies (mAb) against isolated pig Null T cells were derived using a novel twocolour cytofluorometric assay. One (MAC320) identified all blood CD2<superscript>-</superscript> sIg<superscript>-</superscript> 'Null' cells (present at up to ∼6 × 10<superscript>6</superscript>/ml). Another type (MAC319 and MAC318) identified a subset comprising ∼60% or ∼30% of the Null cell population. This percentage appears genetically determined. This subset partially overlapped with a γδ T-cell receptor<superscript>+</superscript> (TcR<superscript>+</superscript>) population which consisted of ∼40% of Null T cells. The antibodies did not react with other leucocyte or lymphocyte populations. In non-reducing conditions, MAC320 precipitated two molecules at ∼270,000-280,000 MW in SDS-PAGE; the larger of which was also precipitated by MAC319 (and MAC318, which binds to the same epitope). Under reducing conditions, MAC320 immunoprecipitated two or three polypeptide chains at ∼130,000-160,000 MW; MAC319 precipitated only the largest of these polypeptides, The large MAC319<superscript>+</superscript> MAC320<superscript>+</superscript> molecule on one subset is removed by bromelain treatment; the smaller MAC319<superscript>-</superscript> MAC320<superscript>+</superscript> molecule on the remaining Null cells is not bromelain sensitive. Several properties of this new antigen complex specific to pig Null T cells show that it is distinct from the ruminant T19 complex. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOCLONAL antibodies
T cells
CYTOFLUOROMETRY
LEUCOCYTES
LYMPHOCYTES
BROMELIN
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 77
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13603923