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Ligation of members of the β1 or the β2 subfamilies of integrins by antibodies triggers eosinophil respiratory burst and spreading.
- Source :
- Immunology; Oct93, Vol. 80 Issue 2, p273-280, 8p
- Publication Year :
- 1993
-
Abstract
- Eosinophils interact with extracellular matrix proteins and endothelial cells through adhesion proteins belonging to the β<subscript>1</subscript> and β<subscript>2</subscript> subfamilies of integrins. Extending previous observations, we found that tumour necrosis factor (TNF) and granulocyte-macrophage colony-stimulating factor stimulated generation of superoxide anion by eosinophils plated on fibronectin-coated surfaces. As studies with adherent neutrophils indicated that TNF might act as activating leucocyte integrins to deliver signals involved in activation of cell functions, we investigated the effects of monoclonal antibodies (mAb) directed against VLA-4 (CD49d/CD29), LFA-I (CD1 la/CDI8), CR3 (CDI lb/ CD 18) or the common β<subscript>2</subscript> subunit (CD 18) on generation of eosinophil toxic oxygen molecules and spreading. We show that cross-linking of members of both the β and the β<subscript>2</subscript> integrin subfamilies triggers eosinophil respiratory burst and spreading. Evidence for the selectivity of anti-integrin mAb effects is derived from thc findings that isotype-matched mAb of other specificities (anti-class I MHC Ag, anti-β<subscript>2</subscript>-microglobulin, anti-CD4) did not trigger eosinophil functions. The findings presented in this paper suggest that integrin-dependent, cosinophil adhesion in sites of allergic reaction may be accompanied by release of toxic oxygen molecules involved in tissue damage. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 80
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13606369