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Long Noncoding RNA Expression Profile in BV2 Microglial Cells Exposed to Lipopolysaccharide.
- Source :
- BioMed Research International; 6/11/2019, p1-9, 9p
- Publication Year :
- 2019
-
Abstract
- Neuropathic pain, which is one of the most common forms of chronic pain, seriously increases healthcare costs and impairs patients' quality of life with an incidence of 7–10% worldwide. Microglia cell activation plays a key role in the progression of neuropathic pain. Better understanding of novel molecules modulating microglia cell activation and these underlying functions will extremely benefit the exploration of new treatment. Recent studies suggested long noncoding RNAs may be involved in neuropathic pain. However, its underlying functions and mechanisms in microglia cell activation remain unclear. To identify the differentially expressed lncRNAs and predict their functions in the progression of microglia cell activation, GSE103156 was analyzed using integrated bioinformatics methods. The expression levels of selected lncRNAs and mRNAs were determined by real-time PCR. In the present study, a total of 56 lncRNAs and 298 mRNAs were significantly differentially expressed. The differentially expressed mRNAs were mainly enriched in NF-kappa B signaling pathway, TNF signaling pathway, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway. The top 10 hub genes were Tnf, Il6, Stat1, Cxcl10, Il1b, Tlr2, Irf1, Ccl2, Irf7, and Ccl5 in the PPI network. Our results showed that Gm8989, Gm8979, and AV051173 may be involved in the progression of microglia cell activation. Taken together, our findings suggest that lots of lncRNAs may be involved in BV2 microglia cell activation in vitro. The findings may provide relevant information for the development of promising targets for the microglial cells activation of neuropathic pain in vivo in the future. [ABSTRACT FROM AUTHOR]
- Subjects :
- RNA analysis
CARRIER proteins
CELLULAR signal transduction
CHEMOKINES
INTERFERONS
INTERLEUKIN-1
INTERLEUKINS
MESSENGER RNA
NEURALGIA
NEUROGLIA
POLYMERASE chain reaction
TRANSCRIPTION factors
TUMOR necrosis factors
DNA-binding proteins
BIOINFORMATICS
LIPOPOLYSACCHARIDES
GENE expression profiling
TOLL-like receptors
SIGNAL peptides
IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 136901289
- Full Text :
- https://doi.org/10.1155/2019/5387407