Back to Search
Start Over
Cyclic peptide: a safe and effective alternative to synthetic aflatoxin B1-competitive antigens.
- Source :
- Analytical & Bioanalytical Chemistry; Jul2019, Vol. 411 Issue 17, p3881-3890, 10p
- Publication Year :
- 2019
-
Abstract
- Aflatoxin B<subscript>1</subscript> (AFB<subscript>1</subscript>) is one of the major mycotoxins, which naturally occurs in food and agricultural products. In this study, a cyclic peptide (CVPSKPGLC) mimicking AFB<subscript>1</subscript> was used to develop a biotinylated peptide–based immunoassay (bp-ELISA) for AFB<subscript>1</subscript> determination. This cyclic peptide was isolated from a commercially available phage-displayed random 7-mer cyclic peptide library, and then synthesized chemically. Instead of phage particles, the peptide was biotinylated and used to detect AFB<subscript>1</subscript> by bp-ELISA, with an IC<subscript>50</subscript> of 0.92 ng/mL, which was approximately 60-fold better than that of phage ELISA. Good recoveries (83–102%) were obtained in spiked rice and corn samples, which were further validated by high-performance liquid chromatography-fluorescence detector. As better sensitivities (0.92–1.21 ng/mL) were obtained by bp-ELISA even using selected anti-AFB<subscript>1</subscript> antibodies prepared previously in laboratory, this cyclic peptide is suitable as a substitute for synthetic competitive AFB<subscript>1</subscript> antigens in food contamination monitoring. [ABSTRACT FROM AUTHOR]
- Subjects :
- AFLATOXINS
ANTIGENS
FOOD contamination
IMMUNOASSAY
MYCOTOXINS
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 411
- Issue :
- 17
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 137184147
- Full Text :
- https://doi.org/10.1007/s00216-019-01862-7