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Characterization and engineering of the Lrp/AsnC family regulator SACE_5717 for erythromycin overproduction in Saccharopolyspora erythraea.
- Source :
- Journal of Industrial Microbiology & Biotechnology; Jul2019, Vol. 46 Issue 7, p1013-1024, 12p, 3 Diagrams, 1 Chart, 4 Graphs
- Publication Year :
- 2019
-
Abstract
- In this work, we found that the Lrp/AsnC family protein SACE_5717 negatively regulated erythromycin biosynthesis in S. erythraea. Disruption of SACE_5717 led to a 27% improvement in the yield of erythromycin in S. erythraea A226. SACE_5717 directly repressed its own gene expression, as well as that of the adjacent gene SACE_5716 by binding to the target sequence 5′-GAACGTTCGCCGTCACGCC-3′. The predicted LysE superfamily protein SACE_5716 directly influenced the export of lysine, histidine, threonine and glycine in S. erythraea. Arginine, tyrosine and tryptophan were characterized as the effectors of SACE_5717 by weakening the binding affinity of SACE_5717. In the industrial S. erythraea WB strain, deletion of SACE_5717 (WBΔSACE_5717) increased erythromycin yield by 20%, and by 36% when SACE_5716 was overexpressed in WBΔSACE_5717 (WBΔSACE_5717/5716). In large-scale 5-L fermentation experiment, erythromycin yield in the engineered strain WBΔSACE_5717/5716 reached 4686 mg/L, a 41% enhancement over 3323 mg/L of the parent WB strain. [ABSTRACT FROM AUTHOR]
- Subjects :
- OVERPRODUCTION
HISTIDINE
TRYPTOPHAN
GENE expression
BIOSYNTHESIS
FAMILIES
Subjects
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 46
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 137276349
- Full Text :
- https://doi.org/10.1007/s10295-019-02178-2