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IL17RC affects the predisposition to thoracic ossification of the posterior longitudinal ligament.
- Source :
- Journal of Orthopaedic Surgery & Research; 7/10/2019, Vol. 14 Issue 1, pN.PAG-N.PAG, 1p, 3 Color Photographs, 2 Black and White Photographs, 2 Charts, 1 Graph
- Publication Year :
- 2019
-
Abstract
- Background: Thoracic ossification of the posterior longitudinal ligament (T-OPLL) can cause thoracic spinal stenosis, which results in intractable myelopathy and radiculopathy. The etiology of T-OPLL is unknown and the condition is difficult to treat surgically. Whole-genome sequencing identified a genetic variant at rs199772854 of the interleukin 17 receptor C (IL17RC) gene as a potentially pathogenic locus associated with T-OPLL. We aimed to determine whether the rs199772854A site mutation causes abnormal expression of the IL17RC in Han Chinese patients with T-OPLL and predict the possible pathogenic mechanisms of T-OPLL. Analyses were performed to determine whether IL17RC is involved in the pathogenicity of T-OPLL. Methods: Peripheral blood and OPLL tissue were collected from a total of 72 patients with T-OPLL disease (36 patients carrying the rs199772854A site mutation in IL17RC and 36 wild-type patients). The expression of IL17RC was analyzed by enzyme-linked immunosorbent assay, reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, and Western blotting. Results: rs199772854A mutation resulted in markedly increased IL17RC gene expression levels in peripheral blood samples and the OPLL tissue obtained following clinical surgery (P < 0.05). Conclusions: The results suggest that the rs199772854A site mutation of IL17RC can significantly increase the expression of IL17RC. The IL17RC gene rs199772854A site polymorphism is a potential pathogenic mutation in T-OPLL disease, which may be associated with the occurrence of T-OPLL. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL receptors
ENZYME-linked immunosorbent assay
GENE expression
GENETIC polymorphisms
IMMUNOHISTOCHEMISTRY
INTERLEUKINS
METAPLASTIC ossification
GENETIC mutation
POLYMERASE chain reaction
POSTOPERATIVE care
THORACIC vertebrae
WESTERN immunoblotting
REVERSE transcriptase polymerase chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 1749799X
- Volume :
- 14
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Orthopaedic Surgery & Research
- Publication Type :
- Academic Journal
- Accession number :
- 137418917
- Full Text :
- https://doi.org/10.1186/s13018-019-1481-6