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Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges.

Authors :
Estes, Mary K.
Ettayebi, Khalil
Tenge, Victoria R.
Murakami, Kosuke
Karandikar, Umesh
Lin, Shih-Ching
Ayyar, B. Vijayalakshmi
Cortes-Penfield, Nicolas W.
Haga, Kei
Neill, Frederick H.
Opekun, Antone R.
Broughman, James R.
Zeng, Xi-Lei
Blutt, Sarah E.
Crawford, Sue E.
Ramani, Sasirekha
Graham, David Y.
Atmar, Robert L.
Source :
Viruses (1999-4915); Jul2019, Vol. 11 Issue 7, p638-638, 1p
Publication Year :
2019

Abstract

Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for infection, knowledge of the site of infection in humans, and advances in the cultivation of human intestinal epithelial cells achieved by developmental and stem cell biologists. The human stem cell-derived enteroid cultivation system recapitulates the multicellular, physiologically active human intestinal epithelium, and allows studies of virus-specific replication requirements, evaluation of human host-pathogen interactions, and supports the pre-clinical assessment of methods to prevent and treat HuNoV infections. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19994915
Volume :
11
Issue :
7
Database :
Complementary Index
Journal :
Viruses (1999-4915)
Publication Type :
Academic Journal
Accession number :
137830081
Full Text :
https://doi.org/10.3390/v11070638