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Altered erythroid-related miRNA levels as a possible novel biomarker for detection of autologous blood transfusion misuse in sport.
- Source :
- Transfusion; Aug2019, Vol. 59 Issue 8, p2709-2721, 13p, 2 Diagrams, 3 Charts, 3 Graphs
- Publication Year :
- 2019
-
Abstract
- <bold>Background: </bold>Autologous blood transfusion (ABT) is a performance-enhancing method prohibited in sport; its detection is a key issue in the field of anti-doping. Among novel markers enabling ABT detection, microRNAs (miRNAs) might be considered a promising analytical tool.<bold>Study Design and Methods: </bold>We studied the changes of erythroid-related microRNAs following ABT, to identify novel biomarkers. Fifteen healthy trained males were studied from a population of 24 subjects, enrolled and randomized into a Transfusion (T) and a Control (C) group. Seriated blood samples were obtained in the T group before and after the two ABT procedures (withdrawal, with blood refrigerated or cryopreserved, and reinfusion), and in the C group at the same time points. Traditional hematological parameters were assessed. Samples were tested by microarray analysis of a pre-identified set of erythroid-related miRNAs.<bold>Results: </bold>Hematological parameters showed moderate changes only in the T group, particularly following blood withdrawal. Among erythroid-related miRNAs tested, following ABT a pool of 7 miRNAs associated with fetal hemoglobin and regulating transcriptional repressors of gamma-globin gene was found stable in C and differently expressed in three out of six T subjects in the completed phase of ABT, independently from blood conservation. Particularly, two or more erythropoiesis-related miRNAs within the shortlist constituted of miR-126-3p, miR-144-3p, miR-191-3p, miR-197-3p, miR-486-3p, miR-486-5p, and miR-92a-3p were significantly upregulated in T subjects after reinfusion, with a person-to-person variability but with congruent changes.<bold>Conclusions: </bold>This study describes a signature of potential interest for ABT detection in sports, based on the analysis of miRNAs associated with erythroid features. [ABSTRACT FROM AUTHOR]
- Subjects :
- AUTOTRANSFUSION of blood
MICRORNA
FETAL hemoglobin
Subjects
Details
- Language :
- English
- ISSN :
- 00411132
- Volume :
- 59
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 137845735
- Full Text :
- https://doi.org/10.1111/trf.15383