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Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae.
- Source :
- AIMS Genetics; 2019, Vol. 6 Issue 3, p46-54, 9p
- Publication Year :
- 2019
-
Abstract
- Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA primers
AXONS
WOUND healing
RNA sequencing
Subjects
Details
- Language :
- English
- ISSN :
- 23771143
- Volume :
- 6
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- AIMS Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 137997883
- Full Text :
- https://doi.org/10.3934/genet.2019.3.46