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Kuijieling-Containing Serum Regulates Th17 and Treg Cell Differentiation by Inhibiting STAT3 Signaling In Vitro.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 8/25/2019, p1-9, 9p, 5 Graphs
- Publication Year :
- 2019
-
Abstract
- Object. To investigate the effect of Kuijieling (KJL) on the balance between T helper 17 (Th17) and regulatory T (Treg) cells in peripheral blood mononuclear cells (PBMC) in vitro and explore the underlying mechanism. Materials and Methods. PBMCs isolated from rats were stimulated with transforming growth factor-β, interleukin (IL)-6, and IL-23 to induce the imbalance of Th17 and Treg cells and were treated with 10, 5, or 2.5% KJL-containing serum. The proportion of Th17 or Treg cells in CD4<superscript>+</superscript> T cells was analyzed by flow cytometry, the concentrations of IL-17, IL-21, and IL-10 were assayed by ELISA, mRNA expressions of retinoic acid-related orphan receptor γt (RORγt), forkhead box protein 3 (Foxp3), and signal transducer and activator of transcription 3 (STAT3) were quantified by PCR, and phosphorylated STAT3 (p-STAT3) was analyzed by flow cytometry. Results. KJL-containing serum decreased the proportion of Th17 cells and increased the proportion of Treg cells in CD4<superscript>+</superscript> T cells, decreased the concentration of IL-17 and IL-21, enhanced the level of IL-10 in the cell culture supernatant, promoted the expression of Foxp3, and inhibited the levels of RORγt, STAT3, and p-STAT3. Conclusion. KJL suppresses the STAT3 pathway to remedy the imbalance between Th17 and Treg cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANIMAL experimentation
CELL differentiation
CELLULAR signal transduction
ENZYME-linked immunosorbent assay
FLOW cytometry
GENE expression
INTERLEUKINS
MEDICINAL plants
CHINESE medicine
MESSENGER RNA
PHOSPHORYLATION
POLYMERASE chain reaction
RATS
T cells
TRANSCRIPTION factors
TRANSFORMING growth factors-beta
TRETINOIN
SIGNAL peptides
IN vitro studies
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 138242358
- Full Text :
- https://doi.org/10.1155/2019/7837989