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Starch-regulated copper–terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging.
- Source :
- Dalton Transactions: An International Journal of Inorganic Chemistry; 9/7/2019, Vol. 21 Issue 17, p13017-13025, 9p
- Publication Year :
- 2019
-
Abstract
- Lysosome visualization is very important for accurate diagnosis of human diseases. However, currently developed lysosome imaging probes usually have poor specificity and are easily quenched, leading to a low signal to noise ratio in lysosome labeling. To resolve this problem, herein, metal–organic framework-based probes of copper–terephthalic acid (CuBDC) are investigated, which show sensitivity to pH and hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>), simultaneously. By self-assembling under the template effect of soluble starch, the particle size of CuBDC can be well controlled for entering into cells and locating lysosomes. Based on the Fenton-like reaction, CuBDC can catalyze the decomposition of H<subscript>2</subscript>O<subscript>2</subscript> into ˙OH, which in turn reacts with CuBDC to generate a stable fluorescent substance. Meanwhile, Cu<superscript>2+</superscript> can be released from CuBDC under acidic conditions for reacting with H<subscript>2</subscript>O<subscript>2</subscript> more thoroughly. And the synthesized CuBDC has a similar attraction to the electrophilic ˙OH at different pH values owing to the residual soluble starch in the particles. The above properties cause CuBDC to have a stable fluorescence signal with low pH values and high H<subscript>2</subscript>O<subscript>2</subscript> concentration, simultaneously. The fluorescence imaging experiments in HeLa cells demonstrate that CuBDC acting as a pH/H<subscript>2</subscript>O<subscript>2</subscript> responsive fluorescent probe holds great promise for lysosome-specific imaging. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14779226
- Volume :
- 21
- Issue :
- 17
- Database :
- Complementary Index
- Journal :
- Dalton Transactions: An International Journal of Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 138294371
- Full Text :
- https://doi.org/10.1039/c9dt02193k