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Starch-regulated copper–terephthalic acid as a pH/hydrogen peroxide simultaneous-responsive fluorescent probe for lysosome imaging.

Authors :
Chen, Jian
Si, Yubing
Liu, Yibiao
Wang, Saisai
Wang, Shijie
Zhang, Ying
Yang, Baocheng
Zhang, Zuling
Zhang, Shouren
Source :
Dalton Transactions: An International Journal of Inorganic Chemistry; 9/7/2019, Vol. 21 Issue 17, p13017-13025, 9p
Publication Year :
2019

Abstract

Lysosome visualization is very important for accurate diagnosis of human diseases. However, currently developed lysosome imaging probes usually have poor specificity and are easily quenched, leading to a low signal to noise ratio in lysosome labeling. To resolve this problem, herein, metal–organic framework-based probes of copper–terephthalic acid (CuBDC) are investigated, which show sensitivity to pH and hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>), simultaneously. By self-assembling under the template effect of soluble starch, the particle size of CuBDC can be well controlled for entering into cells and locating lysosomes. Based on the Fenton-like reaction, CuBDC can catalyze the decomposition of H<subscript>2</subscript>O<subscript>2</subscript> into ˙OH, which in turn reacts with CuBDC to generate a stable fluorescent substance. Meanwhile, Cu<superscript>2+</superscript> can be released from CuBDC under acidic conditions for reacting with H<subscript>2</subscript>O<subscript>2</subscript> more thoroughly. And the synthesized CuBDC has a similar attraction to the electrophilic ˙OH at different pH values owing to the residual soluble starch in the particles. The above properties cause CuBDC to have a stable fluorescence signal with low pH values and high H<subscript>2</subscript>O<subscript>2</subscript> concentration, simultaneously. The fluorescence imaging experiments in HeLa cells demonstrate that CuBDC acting as a pH/H<subscript>2</subscript>O<subscript>2</subscript> responsive fluorescent probe holds great promise for lysosome-specific imaging. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14779226
Volume :
21
Issue :
17
Database :
Complementary Index
Journal :
Dalton Transactions: An International Journal of Inorganic Chemistry
Publication Type :
Academic Journal
Accession number :
138294371
Full Text :
https://doi.org/10.1039/c9dt02193k