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Topical Insulin Modulates Inflammatory and Proliferative Phases of Burn-Wound Healing in Diabetes-Induced Rats.
- Source :
- Biological Research for Nursing; Oct2019, Vol. 21 Issue 5, p473-484, 12p
- Publication Year :
- 2019
-
Abstract
- The healing time of burn wounds depends on surface area and depth of the burn and associated comorbidities. Diabetes mellitus (DM) causes delays in the healing process by extending the inflammatory phase. Treatment with topical insulin can improve the inflammatory phase, restore metabolic dysregulation, and modulate impaired cellular signaling in burn wounds. The objective of this study was to evaluate markers of the inflammatory and proliferative phases of second-degree burns after topical insulin treatment in diabetic rats. Type I DM was induced with streptozotocin in male Wistar rats. The animals' backs were shaved and subjected to thermal burning. Rats were randomized into two groups: control diabetic (DC) and insulin diabetic (DI). At Days 7 and 14 postburn, rats were euthanized, and wound-tissue sections were evaluated by hematoxylin and eosin, Weigert, and Verhöeff staining, immunohistochemistry-paraffin, and enzyme-linked immunosorbent assay. A significant increase in reepithelialization was seen on Days 7 and 14 in DI versus DC rats. On Day 7, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1, and F4/80 expression were increased in DI versus DC rats. On Day 14, MCP-1 expression was decreased and F4/80 increased in DI versus DC rats. On Days 7 and 14, Ki-67, transforming growth factor-β1, vascular endothelial growth factor expression, and formation of elastic fibers were increased in DI versus DC rats. Topical insulin modulates burn-wound healing in diabetic animals by balancing inflammation and promoting angiogenesis and formation of elastic fibers. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL proliferation
ANIMAL experimentation
BURNS & scalds
DIABETES
ENZYME-linked immunosorbent assay
INFLAMMATION
INSULIN
INTERLEUKINS
RATS
RESEARCH funding
STATISTICAL sampling
STAINS & staining (Microscopy)
T-test (Statistics)
CUTANEOUS therapeutics
TUMOR necrosis factors
UREA
WOUND healing
VASCULAR endothelial growth factors
MANN Whitney U Test
ONE-way analysis of variance
Subjects
Details
- Language :
- English
- ISSN :
- 10998004
- Volume :
- 21
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Biological Research for Nursing
- Publication Type :
- Academic Journal
- Accession number :
- 138439880
- Full Text :
- https://doi.org/10.1177/1099800419864443