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Activated integrins identify functional antigen-specific CD8+ T cells within minutes after antigen stimulation.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 6/12/2018, Vol. 115 Issue 24, pE5536-E5545, 10p
- Publication Year :
- 2018
-
Abstract
- Immediate β<subscript>2</subscript>-integrin activation upon T cell receptor stimulation is critical for effective interaction between T cells and their targets and may therefore be used for the rapid identification and isolation of functional T cells. We present a simple and sensitive flow cytometry-based assay to assess antigen-specific T cells using fluorescent intercellular adhesion molecule (ICAM)-1 multimers that specifically bind to activated β<subscript>2</subscript>-integrins. The method is compatible with surface and intracellular staining; it is applicable for monitoring of a broad range of virus-, tumor-, and vaccine-specific CD8<superscript>+</superscript> T cells, and for isolating viable antigen-reacting cells. ICAM-1 binding correlates with peptide-MHC multimer binding but, notably, it identifies the fraction of antigen-specific CD8<superscript>+</superscript> T cells with immediate and high functional capability (i.e., expressing high levels of cytotoxic markers and cytokines). Compared with the currently available methods, staining of activated β<subscript>2</subscript>-integrins presents the unique advantage of requiring activation times of only several minutes, therefore delivering functional information nearly reflecting the in vivo situation. Hence, the ICAM-1 assay is most suitable for rapid and precise monitoring of functional antigen-specific T cell responses, including for patient samples in a variety of clinical settings, as well as for the isolation of functional T cells for adoptive cell-transfer immunotherapies. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
CELL adhesion molecules
INTEGRINS
T cell receptors
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 115
- Issue :
- 24
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 138924195
- Full Text :
- https://doi.org/10.1073/pnas.1720714115