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Development of a ratiometric two-photon fluorescent probe for imaging of hydrogen peroxide in ischemic brain injury.
- Source :
- Analyst; 10/21/2019, Vol. 144 Issue 20, p5965-5970, 6p
- Publication Year :
- 2019
-
Abstract
- Hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>) plays crucial roles in immunological processes and has been revealed to be closely linked to the hypoxic–ischemic process. Thus, it is important to develop a reliable method for monitoring H<subscript>2</subscript>O<subscript>2</subscript> in hypoxic–ischemic brain injury (HIBI). To achieve this, we report on a rationally designed fluorene-based ratiometric two-photon fluorescent probe for H<subscript>2</subscript>O<subscript>2</subscript>, i.e., FH<subscript>2</subscript>O<subscript>2</subscript>. The probe and fluorophore were tested under either one- or two-photon modes, respectively. After reacting with H<subscript>2</subscript>O<subscript>2</subscript>, the relative emission intensity ratio at wavelengths of 390–465 (F<subscript>blue</subscript>) and 500–550 nm (F<subscript>yellow</subscript>) of FH<subscript>2</subscript>O<subscript>2</subscript> had a 6.5-fold increase (F<subscript>yellow</subscript>/F<subscript>blue</subscript>) within 40 min, and the maximal two-photon active cross-section value was detected as 66 GM at a wavelength of 750 nm. The probe exhibited high selectivity, low cytotoxicity, high sensitivity with a detection limit of 0.57 μM, and adequate photostability. After confirming satisfactory sensing performance of the probe in vitro, we were able to monitor H<subscript>2</subscript>O<subscript>2</subscript>in situ in mice with HIBI via two-photon microscopy, which could provide a potential method for clinical diagnosis during the neonatal HIBI process. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 144
- Issue :
- 20
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 138994190
- Full Text :
- https://doi.org/10.1039/c9an01326a