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The Effects of Tetramethylammonium Cation on Oligonucleotide Analysis with Aerolysin Nanopore.
- Source :
- ChemElectroChem; 10/1/2019, Vol. 6 Issue 19, p5086-5089, 4p
- Publication Year :
- 2019
-
Abstract
- Nanopore biosensors, as an extremely sensitive tool, are widely applied to single‐molecule analysis. Compared with the commonly used α‐hemolysin, aerolysin nanopore possess a narrower channel, which distributes the abundant charged residues in the lumen. We replaced the commonly used potassium cations (K+) with tetramethylammonium cations (TMA+) to explore the cation effects. Experimental results demonstrate that the blockage of oligonucleotides in the TMA+ system is distinctly more considerable than that in K+ system. In particular, the blockade currents of Poly(dA)5 are very closed to 0 pA. In addition, the capture rate of oligonucleotides is distinctly increased, and it is speculated that amphipathic TMA+ can enhance the affinity of aerolysin nanopore for oligonucleotides. Furthermore, the estimated effective charges (Zinside) of oligonucleotides inside aerolysin nanopore in two solutions suggest that the screening of K+ on Poly(dA)3, Poly(dA)4, and Poly(dA)5 is more effective than that for TMA+. This report deepens our understanding of the effects of cations in electrolyte. [ABSTRACT FROM AUTHOR]
- Subjects :
- OLIGONUCLEOTIDE analysis
CATION analysis
OLIGONUCLEOTIDES
BIOSENSORS
Subjects
Details
- Language :
- English
- ISSN :
- 21960216
- Volume :
- 6
- Issue :
- 19
- Database :
- Complementary Index
- Journal :
- ChemElectroChem
- Publication Type :
- Academic Journal
- Accession number :
- 139114810
- Full Text :
- https://doi.org/10.1002/celc.201901376