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Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography.
- Source :
- Green Chemistry; 10/21/2019, Vol. 21 Issue 20, p5501-5506, 6p
- Publication Year :
- 2019
-
Abstract
- Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (∼100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 21
- Issue :
- 20
- Database :
- Complementary Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 139115730
- Full Text :
- https://doi.org/10.1039/c9gc01063g