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Migration inhibition of lymph node lymphocytes as an <em>in vitro</em> assay for cell-mediated immunity in the draining lymph nodes of parenterally immunized mice.
- Source :
- Immunology; Oct82, Vol. 47 Issue 2, p357-364, 8p
- Publication Year :
- 1982
-
Abstract
- This paper describes a method for in vitro measurement of specific cell-mediated immunity in the mouse. Animals were immunized parenterally with ovalbumin in Freund's incomplete or complete adjuvant, and a direct migration inhibition assay was performed, using lymphoid cells from the draining lymph nodes. Migration inhibition was found to be antigen specific, correlated with systemic delayed-type hypersensitivity measured in vivo by skin testing, and had a high degree of sensitivity for ovalbumin. The migrating cells were identified as lymphocytes. Lymph node lymphocyte migration inhibition provides a reliable in vitro assay for regional CMl in the mouse. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
LEUCOCYTES
LYMPH nodes
IMMUNITY
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 47
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13937961