POSSIBILITIES OF A QUICK ORIENTATIVE VERIFICATION OF THE ACCURACY OF THE ABSORBANCE MEASUREMENT RESULTS ON ROUTINE DIAGNOSTIC INSTRUMENTS.

Laboratory diagnostics must use equipment that allows to obtain results with acceptable reliability in a short time and for more samples. The laboratory should at least ascertain the accuracy of the instrument with respect to samples of different composition. In our work we evaluated the comparison of the accuracy of absorbance determination on two instruments. We found that with repeated determination of absorbance (n = 11) at 590 nm and 600 nm for the lowest sample dilution coefficient of variation, VK = 43.41 for analyser A and VK = 113.8 for analyser B whereby B did not reach statistical significance of the difference from the blank. Our results suggest that validating these parameters is an important prerequisite for proper laboratory work. [ABSTRACT FROM AUTHOR]