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Effect of <em>Staphylococcus aureus</em> Cowan I bacteria on the mitogenic response of human B-cell subsets.
- Source :
- Immunology; Nov82, Vol. 47 Issue 3, p557-567, 11p
- Publication Year :
- 1982
-
Abstract
- We have made a detailed investigation to determine which of the B-cell subsets could be stimulated by Staphylococcus aureus Cowan 1 bacterium (SpA CoI). B-cell subsets were separated from peripheral blood and tonsil lymphocytes by means of rosette formation with E, EA<subscript>IgG</subscript>, anti-immunoglobulin (Ig) conjugated ox erythrocytes (OE-anti-Ig), and Protein A conjugated OE (OE-Pro A) or by separation on a bovine serum albumin (BSA) discontinuous density gradient. The cells responding to SpA Col included E receptor negative (E<superscript>-</superscript>), C3 receptor positive (C3R<superscript>+</superscript>), and surface Ig positive (SIg<superscript>+</superscript>) B-cell subsets. Among these B-cell subsets, FcR<superscript>-</superscript> cells were more responsive than FcR<superscript>+</superscript> cells. These B-cell subsets responded alone to SpA CoI and significantly proliferated, although they failed to respond alone to pokeweed mitogen (PWM) and Protein A of S. aureus (Protein A). Among the SIg<superscript>+</superscript> B-cell subsets stimulated with SpA CoI, IgM<superscript>+</superscript> and IgG<superscript>+</superscript> B cells showed a marked response and IgA<superscript>+</superscript>, and IgD<superscript>+</superscript> B cells showed much less response. Both Protein A receptor positive (Pro A · R<superscript>+</superscript>) and negative (Pro A · R<superscript>-</superscript>) cells responded well to SpA CoI. Fractionation of B cells on a BSA gradient revealed that comparatively small sized and denser B-cell subsets responded well to SpA CoI. From these criteria, it is suggested that B cells responding to SpA Col are capable of stimulating not only mature B cells, but can also stimulate immature B cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- STAPHYLOCOCCUS aureus
B cells
IMMUNOGLOBULINS
ERYTHROCYTES
PROTEINS
POKEWEED mitogens
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 47
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13942130