Back to Search
Start Over
High and low responsiveness of bovine lymphocytes to <em>Trypanosoma brucei in vitro</em>: lack of correlation with resistance to trypanosomiasis.
- Source :
- Immunology; Jan1985, Vol. 54 Issue 1, p195-203, 9p
- Publication Year :
- 1985
-
Abstract
- Bovine peripheral blood lymphocytes (PBL) were stimulated to proliferate in vitro by live, irradiated or freeze-thawed Trypanosoma brucei, but not by the isolated variant surface glycoprotein. The optimal dose was 10<superscript>5</superscript> trypanosomes per 5 × 10<superscript>5</superscript> lymphocytes in 0.2 ml. Maximal proliferation was at day 5. Of the 98 cattle tested, 36 were high-responders (stimulation indexes 20-104), 49 were iow or non-responders (SI 1-10) and 13 were intermediate. The responder status of individual animals did not change over a period of 1 year, nor did it alter following deliberate trypanosome infection. The stimulation was dependent on macrophage/ monocyte type accessory cells, and this co-operation did not seem to be MHC restricted. Lack of stimulation of non-responder PBL did not appear to be due to the activation of suppressor cells. Accessory cells from non-responder animals could complement PBL from responders, but accessory cells from responders could not complement non-responder PBL. Responsiveness is therefore a characteristic of lymphocytes. Analysis of the surface markers of these lymphocytes or the blast cells generated in culture showed that they were a subpopulation of T cells, possibly T<subscript>H</subscript> cells. Analysis of PBL from 98 animals, which had been selected for trypanoresistance or trypanosensitivity under natural tsetse fly challenge, failed to establish a correlation between resistance and level of lymphocyte stimulation by trypanosomes in vitro. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 54
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13948836