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Complementing urea hydrolysis and nitrate reduction for improved microbially induced calcium carbonate precipitation.

Authors :
Zhu, Xuejiao
Wang, Jianyun
De Belie, Nele
Boon, Nico
Source :
Applied Microbiology & Biotechnology; Nov2019, Vol. 103 Issue 21/22, p8825-8838, 14p
Publication Year :
2019

Abstract

Microbial-induced CaCO<subscript>3</subscript> precipitation has been widely applied in bacterial-based self-healing concrete. However, the limited biogenetic CaCO<subscript>3</subscript> production by bacteria after they were introduced into the incompatible concrete matrix is a major challenge of this technology. In the present study, the potential of combining two metabolic pathways, urea hydrolysis and nitrate reduction, simultaneously in one bacteria strain for improving the bacterial CaCO<subscript>3</subscript> yield has been investigated. One bacterial strain, Ralstonia eutropha H16, which has the highest Ca<superscript>2+</superscript> tolerance and is capable of performing both urea hydrolysis and nitrate reduction in combined media was selected among three bacterial candidates based on the enzymatic examinations. Results showed that H16 does not need oxygen for urea hydrolysis and urease activity was determined primarily by cell concentration. However, the additional urea in the combined medium slowed down the nitrate reduction rate to 7 days until full NO<subscript>3</subscript><superscript>−</superscript> decomposition. Moreover, the nitrate reduction of H16 was significantly restricted by an increased Ca<superscript>2+</superscript> ion concentration in the media. Nevertheless, the overall CaCO<subscript>3</subscript> precipitation yield can be improved by 20 to 30% after optimization through the combination of two metabolic pathways. The highest total CaCO<subscript>3</subscript> precipitation yield achieved in an orthogonal experiment was 14 g/L. It can be concluded that Ralstonia eutropha H16 is a suitable bacterium for simultaneous activation of urea hydrolysis and nitrate reduction for improving the CaCO<subscript>3</subscript> precipitation and it can be studied later, on activation of multiple metabolic pathways in bacteria-based self-healing concrete. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01757598
Volume :
103
Issue :
21/22
Database :
Complementary Index
Journal :
Applied Microbiology & Biotechnology
Publication Type :
Academic Journal
Accession number :
139633052
Full Text :
https://doi.org/10.1007/s00253-019-10128-2