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Asparagine Synthesis during Tobacco Leaf Curing.

Authors :
Bovet, Lucien
Cheval, Cecilia
Hilfiker, Aurore
Battey, James
Langlet, Delphine
Broye, Herve
Schwaar, Joanne
Ozelley, Pierrick
Lang, Gerhard
Bakaher, Nicolas
Laparra, Helene
Goepfert, Simon
Source :
Plants (2223-7747); Nov2019, Vol. 8 Issue 11, p492, 1p
Publication Year :
2019

Abstract

Senescence is a genetically controlled mechanism that modifies leaf chemistry. This involves significant changes in the accumulation of carbon- and nitrogen-containing compounds, including asparagine through the activity of asparagine synthetases. These enzymes are required for nitrogen re-assimilation and remobilization in plants; however, their mechanisms are not fully understood. Here, we report how leaf curing—a senescence-induced process that allows tobacco leaves to dry out—modifies the asparagine metabolism. We show that leaf curing strongly alters the concentration of the four main amino acids, asparagine, glutamine, aspartate, and glutamate. We demonstrate that detached tobacco leaf or stalk curing has a different impact on the expression of asparagine synthetase genes and accumulation of asparagine. Additionally, we characterize the main asparagine synthetases involved in the production of asparagine during curing. The expression of ASN1 and ASN5 genes is upregulated during curing. The ASN1-RNAi and ASN5-RNAi tobacco plant lines display significant alterations in the accumulation of asparagine, glutamine, and aspartate relative to wild-type plants. These results support the idea that ASN1 and ASN5 are key regulators of asparagine metabolism during leaf curing. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22237747
Volume :
8
Issue :
11
Database :
Complementary Index
Journal :
Plants (2223-7747)
Publication Type :
Academic Journal
Accession number :
139789875
Full Text :
https://doi.org/10.3390/plants8110492