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Inhibition of human lymphocyte blastogenesis by C3: the role of serum in the tissue culture medium.
- Source :
- Immunology; Jun82, Vol. 46 Issue 2, p247-252, 6p
- Publication Year :
- 1982
-
Abstract
- Preparations of the third component of human complement (C3) inhibit human lymphocyte blastogenic response to mitogens and antigens when cultured in serum-free medium or in medium supplemented with 5% autologous serum (AS). In contrast, when the culture medium was supplemented with 5% foetal calf serum (FCS), C3 failed to inhibit responses to mitogen (concanavalin A) or to antigen (streptolysin O); some FCS lots allowed stimulation rather than inhibition of the lymphocyte responses. Moreover, when lymphocytes were cultured in serum containing equal amounts of FCS and AS, no inhibition was seen. Our findings may explain previous studies which suggest that C3 enhances or has no effect on lymphocyte responses. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIGENS
MITOGENS
LYMPHOCYTES
BLASTOGENESIS (Embryology)
TISSUE culture
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 46
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13990934