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Quantitative analysis of integrated Eα;d gene expression in C57BL/6 transgenic mice.
- Source :
- Immunology; Dec88, Vol. 65 Issue 4, p589-595, 7p
- Publication Year :
- 1988
-
Abstract
- We performed quantitative analysis of E<subscript>α</subscript><superscript>d</superscript> gene expression in the transgenic mice, created by microinjecting cloned E<subscript>α</subscript><superscript>d</superscript> gene fragments into C57BL/6 fertilized eggs. DNA dot-blot analysis revealed that E<subscript>α</subscript><superscript>d</superscript> gene-introduced transgenic mice (B6E<subscript>α</subscript><superscript>d</superscript> transgenic mice) contain 20 copies per cell of the E<subscript>α</subscript><superscript>d</superscript> gene in their genome. RNA dot-blot analysis revealed that the amount of E<subscript>α</subscript><superscript>d</superscript> mRNAs in B6E<subscript>α</subscript><superscript>d</superscript> transgenic spleen cells is 20-40-fold higher than those in normal BALB/c or (BALB × C578L/6)F<subscript>1</subscript> (CEF<subscript>1</subscript>) spleen cells. However, the amount of E<subscript>α</subscript><superscript>d</superscript> molecules expressed on B6E<subscript>α</subscript><superscript>d</superscript> transgenic spleen cells was similar to that expressed on normal BALB/c of CBF<subscript>1</subscript>, spleen cells on a gene-dose basis. The amount of endogenous E<subscript>α</subscript><superscript>d</superscript> mRNA in the B6E<subscript>α</subscript><superscript>d</superscript> transgenic spleen cells was almost equal to that of normal B6 spleen cells. Since the cell surface I-E molecule is formed by non-covalent association of E. and E<subscript>&beta</subscript> chain, these results suggest that, in spite of the high expression of integrated E<subscript>α</subscript><superscript>d</superscript> gene in the cytoplasm of B6E<subscript>α</subscript><superscript>d</superscript> transgenic mice, the amount of E<subscript>α</subscript><superscript>d</superscript> gene expression on the cell surface is limited by the amount of endogenous E<subscript>α</subscript><superscript>d</superscript> gene products. [ABSTRACT FROM AUTHOR]
- Subjects :
- TRANSGENIC mice
GENE expression
GENETIC regulation
SPLEEN
CELLS
RNA
MESSENGER RNA
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 65
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14003488