Back to Search
Start Over
Different pathways of human T-cell activation revealed by PHA-P and PHA-M.
- Source :
- Immunology; Jan1986, Vol. 57 Issue 1, p55-60, 6p
- Publication Year :
- 1986
-
Abstract
- Antigen-specific T-cell activation is mediated via the CD<subscript>3</subscript>-T<subscript>1</subscript>(antigen receptor) complex. and monoclonal antibodies to both CD<subscript>3</subscript> and T<subscript>1</subscript> cause a rapid rise in intracellular Ca<superscript>2+</superscript>. This calcium mobilization is not inhibited by monoclonal antibodies to CD<subscript>2</subscript>. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD<subscript>2</subscript> expression, and can be blocked in other I cells by anti-CD<subscript>2</subscript> antibodies. A combination of monoclonal antibodies to different epitopes of CD<subscript>2</subscript> causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA- M) induces calcium moblization in cells that lack CD<subscript>2</subscript>, and its effects in other T cells cannot be blocked by anti-CD<subscript>2</subscript> antibodies. The effects of PHA-P and PHA-M arc thus mediated predominantly through different activation pathways. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
MONOCLONAL antibodies
IMMUNOGLOBULINS
CELL culture
EPITOPES
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 57
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14004199