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Bovine T lymphocytes I. GENERATION AND MAINTENANCE OF AN INTERLEUKIN-2-DEPENDENT, CYTOTOXIC T-LYMPHOCYTE CELL LINE.
- Source :
- Immunology; Jan1986, Vol. 57 Issue 1, p131-136, 6p
- Publication Year :
- 1986
-
Abstract
- Primary and secondary bovine allogeneic mixed leucocyte cultures were examined for the generation of antigen-specific cytotoxic leucocytes. While optimal generation of murine and human cytotoxic T lymphocytes typically requires 4-8 days, alloantigen-specific cytotoxic bovine leucocytes were demonstrated consistently only after prolonged incubation periods, optimally found to be about 15 days. Restimulation of long-term bovine mixed leucocyte cultures with the original stimulator population revealed responder cells demonstrating augmented alloantigen-specific lytic activity. When placed into human recombinant interleukin-2, responder cells expanded and required passaging every 3 -4 days. The same was not true of cells placed into interleukin-2-free medium. Cells cultured in interleukin-2-containing medium retained alloantigen specificity after 10 weeks of culture. Moreover, they continued to display total dependence on human, simian or bovine interleukin-2 for growth. [ABSTRACT FROM AUTHOR]
- Subjects :
- LEUCOCYTES
CELL culture
CYTOLOGICAL techniques
INTERLEUKIN-2
T cells
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 57
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14004326