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Antigenic heterogeneity of rat macrophages. A monoclonal antibody reacting only with alveolar but not with other types of macrophages.
- Source :
- Immunology; 4/1/82, Vol. 45 Issue 4, p637-643, 7p
- Publication Year :
- 1982
-
Abstract
- Hybridoma antibodies were produced by fusing spleen cells from mice immunized with rat alveolar macrophages (AM) with P3 × 63Ag8/653 myeloma cells. Some of the hybridomas were processed to achieve monoclonality. Reactivity was tested in an indirect membrane immunofluorescence assay. One of the monoclonal antibodies, called VEP6 antibody, reacted with rat AM but not with peritoneal or splenic macrophages and not with peripheral blood monocytes of this species. It was also unreactive with cells from thymus, bone marrow, non-adherent spleen cells, granulocytes and erythrocytes, but it reacted with thrombocytes. Corynebacterium parvum activated AM were slightly less reactive than 'resident' AM, as determined in a fluorescence activated cell sorter. The VEP6 antigen was studied in polyacrylamide gel electrophoresis after immunoprecipitation from <superscript>125</superscript>I -surface-labelled-macrophage NP40-lysates. Four specific radioactive bands were seen in the precipitates corresponding to molecular weights of 31, 33, 35 and 37,000. A slurred band was seen in this molecular weight range when immunoprecipitates of surface labelled thrombocytes were analysed. This study confirms the heterogeneity of macrophages by showing that certain antigens may be expressed only on a subpopulation of macrophages. [ABSTRACT FROM AUTHOR]
- Subjects :
- HYBRIDOMAS
IMMUNOGLOBULINS
MICE
MACROPHAGES
BONE marrow
ERYTHROCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 45
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14005895