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Cell surface receptors for sulphated polysaccharides: a potential marker for macrophage subsets.
- Source :
- Immunology; Jun86, Vol. 58 Issue 2, p277-284, 8p
- Publication Year :
- 1986
-
Abstract
- The expression of a diverse array of receptors for sulphated polysaccharides on lymphocytes has been demonstrated by Parish & Snowden (1985). This paper presents evidence to suggest that other cell types, namely macrophages, polymorphonuclear leucocytes, mast cells and fibroblasts, can bind similar polysaccharides. Using a rosetting assay and eleven structurally unique polysaccharides, each cell type was observed to bind a characteristic array of these polysaccharides. Analysis of the polysaccharide reactivity of macrophages revealed that BCG-activated and thioglycollate-elicited macrophages express an expanded repertoire of reactivity compared to resident peritoneal macrophages. For example, only thioglycollate-elicited macrophages, but not resident and BCG-activated peritoneal macrophages, reacted with the glycosaminoglycans, chondroitin-4-sulphate, chondroitin-6-sulphate and dermatan sulphate, while both BCG- and thioglycolate-activated, but not resident peritoneal macrophages, bound pentosan polysulphate-coupled sheep erythrocytes. The expression of the receptors for chondroitin-4- and -6-sulphate was observed to be cyclic and peaked at 2 and 5-6 days after thioglycollate treatment. Preliminary analyses of the functional significance of the observed binding of polysaccharides to macrophages revealed that heparin, fucoidan and kappa-carrageenen were specifically endocytosed. However, endocytosis of all other test polysaccharides was not observed. Finally, polysaccharide-coupled sheep erythrocytes were not phagocytosed, even though they interacted strongly with the macrophage surface. The possible relevance of these observations to an inflammatory response and as a means of identifying cellular subsets is discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL receptors
CELLS
POLYSACCHARIDES
BIOPOLYMERS
MACROPHAGES
LEUCOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 58
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14006477