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Biochemical characterization of the T-cell alloantigen RT-6.2.
- Source :
- Immunology; Oct86, Vol. 59 Issue 2, p195-201, 7p
- Publication Year :
- 1986
-
Abstract
- This study presents the partial molecular characterization of the alloantigenic rat T-cell marker RT-6. In contrast to most lymphocyte surface membrane proteins, RT-6 proved to be resistant to short- term (15 mm) solubilization by T X-100 at 4°, but could be efficiently solubilized by long-tent (16 hr) exposure at 4° or short-term (15 mm) exposure at 37°. SDS-PAGE analyses of RT-6.2 immunoprecipitates under non-reducing conditions revealed two bands with apparent molecular weights of 21,000 and 24,000 (25,000 and 28,000 under reducing conditions). Radiolabelled sugars failed to be incorporated metabolically into RT-6.2, neither endoglycosidase-F and 0-glycanase digestion nor NaOH treatment caused any detectable decrease in the molecular weight of RT-6.2, and RT-6.2 failed to bind to any of a series of agarose-coupled lectins with a wide range of sugar-binding specificities. These observations suggest that RT-6.2 may lack carbohydrate moieties. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
ANTIGENS
IMMUNOGLOBULINS
BIOMARKERS
BIOCHEMISTRY
IMMUNITY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 59
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14007192