Back to Search
Start Over
Induction of a β-1,3-D-glucan receptor in P388D1 cells treated with retinoic add or 1,25-dihydroxyvitamin D3.
- Source :
- Immunology; Feb88, Vol. 63 Issue 2, p319-324, 6p
- Publication Year :
- 1988
-
Abstract
- Retinoic add (RA) and 1,25-dihydroxyvitamin D<subscript>3</subscript> (1,25(OH)<subscript>2</subscript>D<subscript>3</subscript>) induce the capability to phagocytose heat-killed yeast (Y) (Saccharomyces cereviciae) in P388D1 cells. Y phagocytosis is specifically inhibited (100%) by particulate and soluble β-1,3-D-glucan. Other polysaccharides, such as agarose, dextran and dextran sulphate, are not inhibitory. The inhibitory capacity of mannan was totally abrogated by treatment with β-glucanase, suggesting that its activity is derived from a residual β-glucan structure. Partial hydrolysis of glucan particles with formic acid yielded soluble glucan that was fractionated according to size. Glucan¹, glucan² and glucan³ had an average chain length of 34, 23.5 and 15.5 glucose units, respectively. Fifty percent inhibition of Y phagocytosis by RA-P388D1 cells was attained at <0.02 μg/ml (∼ 2 nM) glucan¹ and at 1.1 μg/ml glucan³. A further decrease in chain length (≦ 12.6) resulted in oligomers of marginal inhibitory activity. Preincubation of RA- and 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript>-P388D1 cells with glucan¹ for 30 seconds to 5 min, at 4° or 37°, followed by washes with buffer, sufficed to bring about 85-95% inhibition of Y phagocytosis. Recovery of the phagocytic capability was time dependent and required protein synthesis, suggesting a glucan¹-induced removal of membrane receptors. The results suggest that recognition and ingestion of Y by RA- or 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript>-treated P388D1 cells depends almost exlusively on a β-glucan-specific receptor. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 63
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14012559