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Mechanism of action of a Db -specific helper clone and factor in cytotoxic responses to alloantigens.
- Source :
- Immunology; Jun87, Vol. 61 Issue 2, p151-157, 7p
- Publication Year :
- 1987
-
Abstract
- The mechanism by which a D<superscript>b</superscript>-specific helper clone (clone 9) and a hybridoma-derived D<superscript>b</superscript>-especific helper factor, referred to as ASHF, induced cytotoxic T lymphocyte (CTL) responses to alloantigens was investigated. Clone 9 or ASHF helped CBA thymocytes to produce CTL against B6 (H-2<superscript>b</superscript>), but not D2 (H-2<superscript>d</superscript>), alloantigens. However, when BDF1 (H-<superscript>b,d</superscript>) spleen cells or an equal mixture of B6 or D2 spleen cells were used as stimulator cells, CTL responses to both B6 and D2 were induced. This suggested that clone 9 cells or ASHF could induce the production of long-ranged, non-antigen-specific helper factor(s) in B6-stimulated cultures. One of these long-ranged factors produced in B6-stimulated cultures was found to be interleukin-2 (IL-2). Thus, clone 9, which is a non-IL-2 producer, increased the production of IL-2 by irradiated B6 spleen cells and by CBA anti-B6 cultures by 4·7- and 5·7-fold, respectively. ASHF did not increase the amount of IL-2 produced by irradiated B6 spleen cells but increased the amount of IL-2 produced in CBA anti-B6 cultures by 3·8-fold. Clone 9 cells or ASHF did not increase IL-2 production in D2-stimulated cultures. Upon stimulation with concanavalin A or antigen, clone 9 cells also produced a non-antigen-specific helper factor. This factor (IL-X) synergized with excess human recombinant IL.2 (rIL-2) in the polyclonal activation of BDF1 or D2 CTL precursors. A model that involves the participation of ASHF, clone 9 cells, IL-2 and IL-X in the induction of a cytotoxic response is proposed. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
LYMPHOCYTES
CELL-mediated cytotoxicity
ANTIGENS
IMMUNITY
CELL culture
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 61
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14014101