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Heterogeneity in the activation requirements of T cells stimulated by phytohaemagglutinin.
- Source :
- Immunology; Jun87, Vol. 61 Issue 2, p167-172, 6p
- Publication Year :
- 1987
-
Abstract
- Data are presented showing that resting T cells proliferated in response to phytohaemagglutinin (PHA) provided that either sheep erythrocytes (SRC) or interleukin-2 (IL-2) was added. Proliferation requiring PHA and SRC was inhibited by anti-CD2 monoclonal antibody (OKT11) and anti-CD3 monoclonal antibody (OKT3). These monoclonal antibodies only partially inhibited IL-2 production stimulated by PHA and SRC, and added IL-2 did not restore proliferation in these cultures. Low concentrations of cyclosporine inhibited both proliferation and IL-2 production (ED<subscript>50</subscript>=12 and 9 ng/ml, respectively) and this inhibition was partially relieved in the presence of added IL-2 (ED<subscript>50</subscript>= 110 ng/ml). In contrast, proliferation stimulated by PHA with exogenous IL-2 was not inhibited in the presence of OKT11, and moderate concentrations of cyclosporine were required to inhibit proliferation (ED<subscript>50</subscript>= 49 ng/ml). Evidence was obtained, from analysis of cultures at limiting dilution, to suggest that the different requirements for stimulation of PHA-responsive T cells reflected different T-cell subpopulations. The majority of PHA-responsive cells proliferated in the presence of PHA and SRC, whereas only a minority proliferated in the presence of PHA and exogenous IL-2. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 61
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14014116