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Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis.
- Source :
- Chinese Journal of Integrative Medicine; Dec2019, Vol. 25 Issue 12, p939-947, 9p
- Publication Year :
- 2019
-
Abstract
- Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 25
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 140155966
- Full Text :
- https://doi.org/10.1007/s11655-018-3003-5