Permeability of membranes in the liquid ordered and liquid disordered phases.

The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (L_o) phases of three-component lipid bilayers to examine permeability. Molecular dynamics simulations of L_o phase dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), and cholesterol show that oxygen and water transit a leaflet through the DOPC and cholesterol rich boundaries of hexagonally packed DPPC microdomains, freely diffuse along the bilayer midplane, and escape the membrane along the boundary regions. Electron paramagnetic resonance experiments provide critical validation: the measured ratio of oxygen concentrations near the midplanes of liquid disordered (L_d) and L_o bilayers of DPPC/DOPC/cholesterol is 1.75 ± 0.35, in very good agreement with 1.3 ± 0.3 obtained from simulation. The results show how cellular rafts can be structurally rigid signaling platforms while remaining nearly as permeable to small molecules as the L_d phase. Increasing evidence supports the existence of ordered nanodomains (or rafts) in cholesterol rich cell membranes. Here authors present molecular dynamics simulations and EPR experiments to monitor permeation of oxygen and water through membranes in the liquid ordered and liquid disordered phases. [ABSTRACT FROM AUTHOR]