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Trichosanthes tricuspidata Lour. Methanol Extract Exhibits Anti-Inflammatory Activity by Targeting Syk, Src, and IRAK1 Kinase Activity.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 12/16/2019, p1-15, 15p, 1 Chart, 9 Graphs
- Publication Year :
- 2019
-
Abstract
- Trichosanthes tricuspidata Lour., also known as T. palmata Roxb, T. bracteata Lam., T. puber Blume, and Modecca bracteata, is a vine belonging to the Cucurbitaceae family (English name: redball snake gourd). Distributed in China, South and East Asia, and tropical Australia, it has been traditionally used as a medicinal plant for its antifever, laxative, anthelmintic properties and for migraine treatment. In this paper, we examined the effects of Trichosanthes tricuspidata Lour. ethanol extract (Tt-ME) in vitro and in vivo. To confirm the effects of Tt-ME on inflammatory responses, we conducted experimental analyses including level of nitric oxide (NO) production, RT-PCR, and immunoblotting and using a HCl/EtOH-induced gastritis animal model. Tt-ME attenuated the release of NO and decreased mRNA levels of inducible NO synthase (iNOS), TNF-α, and IL-6 in lipopolysaccharide- (LPS-) induced macrophages in a concentration-dependent manner. Tt-ME time-dependently suppressed nuclear translocation of nuclear factor kappa B (NF-κB) subunits p50 and p65, activator protein (AP-1) subunits c-Fos and c-Jun, and STAT3 transcriptional activity by inhibiting nuclear translocation of p50, p65, c-Fos, c-Jun, and STAT3. Tt-ME significantly downregulated NF-κB, MAPK, and JAK2 signaling by targeting Syk, Src, and IRAK1 protein kinases. Furthermore, matrix metalloproteinase-9 (MMP-9) expression and cell migration were observed to be downregulated by Tt-ME in LPS-activated macrophages. In vivo studies on Tt-ME also produced similar trends in Hcl/EtOH-induced gastritis mouse models by inhibiting proinflammatory cytokines and the inflammatory signaling pathway. Our results strongly suggest that Tt-ME exerted anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory disease. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANIMAL experimentation
CELLULAR signal transduction
CYTOKINES
ETHANOL
GASTRITIS
HYDROCHLORIC acid
IMMUNOBLOTTING
INFLAMMATORY mediators
INTERLEUKINS
MACROPHAGES
MELONS
MESSENGER RNA
METHANOL
MICE
NITRIC oxide
POLYMERASE chain reaction
PROTEIN kinases
PROTEIN-tyrosine kinases
TRANSCRIPTION factors
TUMOR necrosis factors
DNA-binding proteins
NITRIC-oxide synthases
PLANT extracts
REVERSE transcriptase polymerase chain reaction
LIPOPOLYSACCHARIDES
IN vitro studies
IN vivo studies
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 140476271
- Full Text :
- https://doi.org/10.1155/2019/6879346