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CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in <em>Trypanosoma congolense</em>-infected cattle.
- Source :
- Immunology; Oct97, Vol. 92 Issue 2, p226-233, 8p
- Publication Year :
- 1997
-
Abstract
- Mice infected with African trypanosomes produce exceptionally large amounts of serum IgM, a major part of which binds to non-trypanosome antigens such as trinitrophenol and single-strand DNA. In this paper, we describe that in cattle infected with Trypanosoma congolense and T. vivax, similar antibodies are found, although they bind mainly to protein antigens, such as β-galactosidase, ovalbumin and ferritin. The parasite non-specific IgM antibodies appear around the same time as the parasite-specific antibodies, but their origin and function are not clear. We tested the hypothesis that CD5<superscript>+</superscript> B cells (or B-1 cells), which increase during trypanosome infections in cattle, are responsible for production of antibodies to non-trypanosome antigens. Splenic CD5<superscript>+</superscript> and CD5<superscript>-</superscript> B cells from infected cattle were sorted and tested in a single cell blot assay. The numbers of immunoglobulin-secreting cells were similar in both B-cell populations. However, antibodies with reactivity for non-trypanosome antigens were significantly more prevalent in the CD5<superscript>+</superscript> B-cell fraction and were exclusively IgM. The preference for production of these antibodies by CD5<superscript>+</superscript> B cells and the expansion of this subpopulation during infections in cattle, strongly suggest that CD5<superscript>+</superscript> B cells are the main source of trypanosome non-specific antibodies. We propose that these antibodies are natural, polyreactive antibodies that are predominantly secreted by CD5<superscript>+</superscript> B cells. Since B-1 cells are up-regulated in many states of immune insufficiency, the immunosuppression associated with trypanosome infections may be responsible for the increase of this subset and the concomitant increase in trypanosome non-specific antibodies. [ABSTRACT FROM AUTHOR]
- Subjects :
- B cells
IMMUNOGLOBULINS
TRYPANOSOMA
CATTLE diseases
IMMUNOGLOBULIN M
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 92
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14051682
- Full Text :
- https://doi.org/10.1046/j.1365-2567.1997.00330.x