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Inhibitors of dipeptidyl peptidase IV induce secretion of transforming growth factor-β1 in PWM-stimulated PBMC and T cells.
- Source :
- Immunology; Jul97, Vol. 91 Issue 3, p354-360, 7p
- Publication Year :
- 1997
-
Abstract
- Various studies have shown that the membrane ectoenzyme dipeptidyl peptidase IV (DP IV; CD26), expressed on T, natural killer (NK) and B cells in the immune system, is involved in the regulation of DNA synthesis and cytokine production. We show that the specific DP IV inhibitors Lys[Z(NO<subscript>2</subscript>)]-thiazolidide, Lys[Z(NO<subscript>2</subscript>)]-piperidide, and Lys[Z(NO<subscript>2</subscript>)]-pyrrolidide inhibit DNA synthesis as well as production of interleukin-2 (IL-2), IL-10, IL-12, and interferon-γ (IFN-γ) of pokeweed mitogen (PWM)-stimulated purified T cells. Most importantly, these inhibitors induce a three- to fourfold increased secretion of latent transforming growth factor-β<subscript>1</subscript>(TGF-β<subscript>1</subscript>) by PWM-stimulated peripheral blood mononuclear cells (PBMC) and T cells, as measured with a specific TGF-β<subscript>1</subscript> enzyme-linked immunosorbent assay and in the Mv1Lu bioassay. As we could demonstrate previously, TGF-β<subscript>1</subscript> exhibits the same inhibitory effects as DP IV inhibitors on DNA synthesis and cytokine production (Cytokine 1994, 6, 382–8; J Interferon Cytokine Res 1995, 15, 685–90). A neutralizing chicken anti-TGF-β<subscript>1</subscript> antibody was capable of abolishing the DP IV inhibitor-induced suppression of DNA synthesis of PWM-stimulated PBMC and T cells. These data suggest that TGF-β<subscript>1</subscript> might have key functions in the molecular action of DP IV/CD26 in regulation of DNA synthesis and cytokine production. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 91
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14054386
- Full Text :
- https://doi.org/10.1046/j.1365-2567.1997.d01-2258.x