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Electrochemiluminescence immunoassay for the N-terminal pro-B-type natriuretic peptide based on resonance energy transfer between a self-enhanced luminophore composed of silver nanocubes on gold nanoparticles and a metal-organic framework of type MIL-125
- Source :
- Microchimica Acta; Dec2019, Vol. 186 Issue 12, p1-8, 8p
- Publication Year :
- 2019
-
Abstract
- The N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a marker of heart failure. A novel sandwich type electrochemiluminescence (ECL) immunoassay is described for the NT-proBNP. The method is based on ECL resonance energy transfer (RET) between silver nanocubes that were covered with semicarbazide-modified gold nanoparticles (AgNC-sem@AuNPs) as the donor, and a Ti(IV)-based metal-organic framework of type MIL-125 as the acceptor. The ECL signal was strongly amplified by increasing the luminous efficiency. ECL-RET occurs due to the partial overlap between the ECL emission of the AgNC-sem@AuNPs (emission wavelength at 470 nm to 900 nm) and the visible absorption spectrum of MIL-125 (absorption wavelength at 406 nm to 900 nm). This results in the quenching of ECL. The AgNC-sem@AuNPs were placed on the electrode. The antibody was immobilized on AgNC-sem@AuNPs via Au-NH<subscript>2</subscript> bond, and MIL-125 was utilized as a label for the secondary antibody. The assay works in the 0.25 pg mL<superscript>−1</superscript> to 100 ng mL<superscript>−1</superscript> concentration range and has a 0.11 pg mL<superscript>−1</superscript> lower detection limit (at S/N = 3). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 186
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 140622614
- Full Text :
- https://doi.org/10.1007/s00604-019-3969-5