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Derivation of T-cell receptor α-chain double expresser lines from normal murine mature T cells.
- Source :
- Immunology; Feb96, Vol. 87 Issue 2, p205-212, 8p
- Publication Year :
- 1996
-
Abstract
- Because the T-cell receptor (TCR) α-chain locus is known to lack allelic exclusion of rearrangements, and as a recent report revealed the existence of α-chain double expressers among normal human peripheral blood lymphocytes (PBL), the possible existence of TCR α-chain double expressers among mature routine T cells was examined. Although two-colour staining analysis of normal T-cell populations did not immediately reveal recognizable clusters of Vet double expressers, alternative/n vitro stimulations of normal murine T cells with antibodies to two different TCR Vα chains reproducibly induced TCR α-chain double-expresser lines. TCR complexes with different α-chains on such T cells were both shown to be functional. The cell lines were heterogeneous with respect to Vβ usage and the ratio of the expressed amounts of the two α-chains on the surface. The ratio of the two expressed α-chains was found to be very stable over a long period of time. These results are consistent with the earlier report on α-chain double expressers among human T cells and also show normal occurrence of TCR α-chain double expressers in routine T-cell populations. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cell receptors
T cells
LYMPHOCYTES
GENES
IMMUNOGLOBULINS
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 87
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14068768
- Full Text :
- https://doi.org/10.1046/j.1365-2567.1996.471534.x