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Discovery and characterization of a stable lipase with preference toward long-chain fatty acids.
- Source :
- Biotechnology Letters; Jan2020, Vol. 42 Issue 1, p171-180, 10p
- Publication Year :
- 2020
-
Abstract
- Objectives: To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database. Results: Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases exhibiting 97%, 55%, and 35% identities with CALA, respectively, were selected for overexpression and characterization. Lipase, PhLip from Pseudozyma hubeiensis SY62 showed highest activity towards long-chain fatty acids, and showed maximum activity at pH 9.0 and 60 °C, and stability between 40 and 50 °C for 4 h and at pH 7–10 for 12 h. Enzymatic hydrolysis of Mucor circinelloides WJ11 oils by PhLip was about twofold higher than that by CALA, with respect to hydrolysis of long-chain fatty acids. Besides, fatty acids with 18 carbons, including oleic acid, linoleic acid, and linolenic acid, were preferred as substrates. Conclusion: The current investigation discovered a stable lipase PhLip with long-chain fatty acids preference. PhLip may be a potential candidate for producing polyunsaturated fatty acids from natural oils. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 42
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 141004026
- Full Text :
- https://doi.org/10.1007/s10529-019-02765-4