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Immunogenicity of Fusion Protein of Cholera Toxin B Subunit-Porphyromonas gingivalis 53-kDa Minor Fimbrial Protein Produced in Nicotiana benthamiana.
- Source :
- Biotechnology & Bioprocess Engineering; Nov2019, Vol. 24 Issue 6, p954-963, 10p
- Publication Year :
- 2019
-
Abstract
- Porphyromonas gingivalis induces destruction of periodontal tissues that surround and support the teeth, contributing to the development of periodontitis, which eventually results in tooth loss in adults. A 53-kDa protein of P. gingivalis is a major subunit variant protein of minor fimbriae (Mfa1), and is reported to be highly immunogenic and considered as a potential vaccine candidate. The gene encoding 53-kDa protein, was divided into three segments, and each DNA segment was fused to the gene coding for cholera toxin B subunit (CTB) to improve gut mucosal immune responses. The ctb-mfal fusion genes were expressed in the leaf tissues of Nicotiana benthamiana using agroinfiltration. Immunoblot analysis revealed that CTB-Mfa1 fusion proteins were produced in the agroinfiltrated leaves. The interaction of the plant-produced CTB-Mfa1 fusion proteins with G<subscript>M1</subscript>-ganglioside, which acts as the binding site for native CTB, was confirmed by G<subscript>M1</subscript>-ELISA. Mice immunized orally with the agroinfiltrated leaf powder containing the CTB-Mfa1 fusion proteins elicited serum IgG and fecal IgA antibodies to CTB and Mfa1. These results suggest that CTB-Mfa1 fusion proteins produced in plants can be used as an oral vaccine to control P. gingivalis infection-associated periodontitis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12268372
- Volume :
- 24
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Biotechnology & Bioprocess Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 141026407
- Full Text :
- https://doi.org/10.1007/s12257-019-0175-6