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TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140.
- Source :
- Cancer Genomics & Proteomics (1109-6535); Jan/Feb2020, Vol. 17 Issue 1, p23-33, 11p
- Publication Year :
- 2020
-
Abstract
- Background/Aim: Colorectal cancer (CRC) cells secrete inflammatory cytokines that affect CRC progression. The aim of the present study was to determine if micro-RNA-140(miR-140) regulates inflammatory cytokine secretion induced by lipopolysaccharide (LPS) in colorectal cancer cells by targeting tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). Materials and Methods: Fifty fresh coloncancer specimens and normal colorectal tissues were collected from patients with CRC and tested for the expression miR-140. Human CRC cell lines SW480 and HCT116 were treated with various concentrations and times with LPS. miR-140 and mRNA expression of potentially related genes were analyzed by qPCR. Protein expression was analyzed using western blot or ELISA. Overexpression plasmids with pcDNA3.1-TRAF6, pGL4.10-wtTRAF6 and pGL4.10-mutTRAF6 were constructed. miRNA target gene prediction and a dual luciferase assay were used to analyze miR-140-targeted TRAF6. Results: miR-140 expression was up-regulated in CRC tissues. In CRC cells, LPS could increase miR-140 expression in a time- and concentrationdependent manner. LPS increased inflammatory cytokine mRNA expression levels in SW480 and HCT116 human colon-cancer cells. miRNA-140 suppressed TRAF6 expression via targeting the 3 UTR. TRAF6 affected miR-140-mediated inflammatory cytokine expression of SW480 and HCT116 cells under LPS treatment. Conclusion: miR-140 regulates inflammatory cytokine secretion of LPS-induced colorectal cancer cells by targeting TRAF6. [ABSTRACT FROM AUTHOR]
- Subjects :
- TUMOR necrosis factor receptors
COLORECTAL cancer
CANCER cells
CYTOKINES
SECRETION
Subjects
Details
- Language :
- English
- ISSN :
- 11096535
- Volume :
- 17
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Cancer Genomics & Proteomics (1109-6535)
- Publication Type :
- Academic Journal
- Accession number :
- 141070354
- Full Text :
- https://doi.org/10.21873/cgp.20164