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Cultivation of Fungi in Synthetic and Semi-Synthetic Liquid Medium II. Immunochemical Properties of the Antigenic and Allergenic Extracts.
- Source :
- Allergy; Nov85, Vol. 40 Issue 8, p592-598, 7p
- Publication Year :
- 1985
-
Abstract
- Four allergologically important fungi, viz. <em>Aspergillus fumigatus, Penicillium notalum Alternaria altermata</em>, and <em>Cladosporium herbarum</em> were cultured in a liquid synthetic me with or without addition of 0.1 % yeast extract (YE). After 10 and 28 days of cultivation, immunochemical properties of the fungal extracts, characterised by precipitation pattern and IgG- and IgE-binding capacity, were studied. In pure synthetic medium no large differences were observed in number of precipitates, as measured by DID, among the four antigenic fractions of each fungus (metabolic and mycelial antigens of both early and late phase cultures). Addition of YE to the growth medium hardly changed the number of precipitates and generally caused a decrease in lgG-binding capacity of the extracts. In contrast to these observations were the findings with the IgE-binding capacities of the fungal extracts as measured by RAST. Most allergenic fractions demonstrated an increase (sometimes strong) in lgE-binding capacity after YE was added to the growth medium. It is concluded that the time of cultivation influences the immunochemical characteristics of fungal extracts and that mycelial as well as metabolic antigens of both early and late phase cultures should be used in order to obtain a wide spectrum of allergens in extracts used for diagnostic purposes. [ABSTRACT FROM AUTHOR]
- Subjects :
- FUNGI
IMMUNOCHEMISTRY
IMMUNITY
ANTIGENS
ASPERGILLUS fumigatus
IMMUNOGLOBULINS
Subjects
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 40
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 14132140
- Full Text :
- https://doi.org/10.1111/j.1398-9995.1985.tb00888.x