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Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae).
- Source :
- Ecotoxicology; Jan2020, Vol. 29 Issue 1, p35-44, 10p
- Publication Year :
- 2020
-
Abstract
- Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdαE7 in the two strains revealed that four non-synonymous mutations (Trp<superscript>251</superscript>-Leu, Asp<superscript>273</superscript>-Glu, Ala<superscript>365</superscript>-Val, and Ile<superscript>396</superscript>-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdαE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies. [ABSTRACT FROM AUTHOR]
- Subjects :
- HOUSEFLY
MUSCIDAE
DIPTERA
SINGLE nucleotide polymorphisms
Subjects
Details
- Language :
- English
- ISSN :
- 09639292
- Volume :
- 29
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Ecotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 141432608
- Full Text :
- https://doi.org/10.1007/s10646-019-02125-y