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Towards the Production of mcl-PHA with Enriched Dominant Monomer Content: Process Development for the Sugarcane Biorefinery Context.
- Source :
- Journal of Polymers & the Environment; Mar2020, Vol. 28 Issue 3, p844-853, 10p
- Publication Year :
- 2020
-
Abstract
- The production of short-chain-length polyhydroxyalkanoates (scl-PHAs) in a sugarcane biorefinery setting has been demonstrated to be an effective strategy to reduce production costs. Medium-chain-length PHA (mcl-PHA) have elastomeric properties and are more suitable for high value-added applications, but its industrial production is not yet established. Mcl-PHA synthesis occurs via different metabolic routes and thus requires distinct microorganisms and substrates compared to scl-PHA. In the present study, sucrose-derived carbohydrates were evaluated as co-substrates for the production of mcl-PHA from decanoic acid (DA). Fermentation strategies were investigated to produce mcl-PHA with enriched dominant monomer content, which is desirable for commercial applications. The mcl-PHA production was investigated in carbon-limited, fed-batch fermentations with wild-type and β-oxidation knockout mutant strains of Pseudomonas putida KT2440. The experimental results demonstrated that a mixture of glucose and fructose was a suitable co-feed with DA for mcl-PHA production, yielding equivalent results to those obtained with starch-derived glucose, a more traditional feedstock for PHA production. The use of a β-oxidation-impaired strain was essential to attain high dominant monomer content. A near-homopolymeric mcl-PHA was produced under exponential feeding, containing 99 mol% of 3-hydroxydecanoate. This work demonstrates the potential for near-homopolymeric mcl-PHA production in a sugarcane biorefinery, using hydrolyzed sucrose and DA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15662543
- Volume :
- 28
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Polymers & the Environment
- Publication Type :
- Academic Journal
- Accession number :
- 141727094
- Full Text :
- https://doi.org/10.1007/s10924-019-01637-2