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Factors affecting the quality of cytochrome C prepared by biological separation.
- Source :
- Chinese Journal of Bioprocess Engineering; 2019, Vol. 17 Issue 6, p569-575, 7p
- Publication Year :
- 2019
-
Abstract
- Cytochrome C is a biochemical drug widely used in clinical treatment. To improve the quality of cytochrome C, we studied the factors influencing the quality of cytochrome C in the preparation process. The purity of cytochrome C prepared by different processes was analyzed by SDS-PAGE, and the 2. 5x10<superscript>4</superscript> protein in SDS-PAGE was analyzed and identified by LC-MS/MS. The results of SDS-PAGE electrophoresis show that the sample of cytochrome C prepared by traditional process using trichloroacetic acid (TCA) precipitator contained not only 1. 22 x 10<superscript>4</superscript> cytochrome C, but also a protein molecule of about 2. 5 x 10<superscript>4</superscript>. Cytochrome C samples prepared by the new process without trichloroacetic acid precipitator only had 1.22 x 10<superscript>4</superscript> cytochrome C, but no 2. 5 x 10<superscript>4</superscript> protein was found. The LC-MS/MS analysis and identification result of the 2. 5 x 10<superscript>4</superscript> protein in the SDS-PAGE map show that the coverage between this protein and cytochrome C (susscrofa) was 76%. Combining with its molecular weight, it can be proved that this protein is cytochrome C dimer. TCA precipitator during the preparation of cytochrome C easily leads to the formation of cytochrome C dimer. Therefore, TCA is one of the key factors affecting the quality of cytochrome C. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 16723678
- Volume :
- 17
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Bioprocess Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 141939773
- Full Text :
- https://doi.org/10.3969/j.issn.1672-3678.2019.06.004